Journal: iScience

Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

doi: 10.1016/j.isci.2019.01.030

Figure Lengend Snippet: List of Candidate Beneficial Bacteria Used to Mono-Associate Germ-free Drosophila and Screened for Their Capacity to Protect Drosophila against Paraquat-Induced Death or Paraquat-Induced Intestinal Damage in a “Smurf” Assay.

Article Snippet: Lactobacillus fermentum , ATCC 9338.

Techniques: Bacteria

Journal: iScience

Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

doi: 10.1016/j.isci.2019.01.030

Figure Lengend Snippet: Lactococcus lactis Subsp. Cremoris ATCC 19257 Confers Potent Cytoprotective Effects in the Drosophila Intestine in Response to Oxidative Challenge (A) Graphical representation of experiments conducted to generate data presented in (B–D). (B) Relative survival of germ-free adult Drosophila gnotobiotically mono-colonized with the indicated lactic acid bacteria in response to paraquat challenge. Note only significantly enhanced survival of flies mono-associated with L . lactis subsp. cremoris ATCC 19257 (log rank test for germ-free (g-f) versus g-f + L . lactis subsp. cremoris , p ≤ 0.0006, n = 50), or mono-colonized with Lactobacillus rhamnosus GG (log rank test for g-f versus g-f + L . rhamnosus GG p ≤ 0.0202, n = 50). (C) TUNEL analysis of posterior midgut tissues dissected from Drosophila described in (A) at 24 h following paraquat challenge. Note the presence of TUNEL-positive cells in the Posterior 3 (P3) section of the midgut in response to paraquat. (D) Numeration of TUNEL-positive cells per midgut examined in (C). Data are represented as mean ± SEM. Nonparametric unpaired t test ***p < 0.001, n = 10. (E) Example of adult Drosophila used to assess gut permeability via a Smurf assay. Germ-free adult Drosophila flies were gnotobiotically mono-colonized with the indicated lactic acid bacteria for 3 days. Thereafter, flies were subjected to a paraquat solution (spiked with inert blue food dye), which induces a leaky gut phenotype within 24–48 h. Flies that have a leaky gut are identified by exhibiting a visible blue color throughout their hemocoel within the body cavity. (F) Numeration of germ-free flies with visible blue color throughout their hemocoel as described in (D), in response to feeding of the indicated bacteria for 5 days, followed by being subjected to 17.5% paraquat solution and inert blue dye for up to 72 h. Note significantly fewer flies with visible blue color throughout their hemocoel following pretreatment with L . lactis subsp. cremoris ATCC 19257. Data are represented as mean ± SEM. Nonparametric unpaired t test ***p < 0.001, n = 50.

Article Snippet: Lactobacillus fermentum , ATCC 9338.

Techniques: Bacteria, TUNEL Assay, Permeability

Journal: iScience

Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

doi: 10.1016/j.isci.2019.01.030

Figure Lengend Snippet: Ingestion of Lactococcus Lactis Subsp. cremoris Is Cytoprotective Against Radiological Insult in Mice. A) Survival of 6-week-old C57BL/6 fed the indicated bacteria daily for 3 days followed 12 Gy irradiation insult. Note significantly enhanced survival of only mice treated before irradiation for three days with L. lactis subsp. cremoris ATCC 19257 (Log-Rank test for untreated vs. L. lactis subsp. cremoris treated, P=0.0006, n=5), or with L. rhamnosus GG (Log-Rank test for untreated vs. L. rhamnosus GG P=0.0202, n=5). (B) Weight of mice described in (A) measured daily after irradiation insult. Nonparametric unpaired t-test of bacterial L. lactis subsp. cremoris ATCC 19257 treated against untreated mice. **=P<0.01, ***=P<0.001, ****=P<0.0001, n=5. (C) Survival of 6-week-old C57BL/6 fed the indicated bacteria daily for 3 days followed 12 Gy irradiation insult. Analysis by Log-Rank test for untreated vs. bacterial treated revealed no significant differences in survival in mice treated Lactobacillus gasseri ATCC 19992, or with Lactobacillus acidophilus ATCC 4356. (D) Weight of mice described in (C) measured daily after irradiation insult. Nonparametric unpaired t-test of bacterial treated against untreated mice revealed no significant differences. (E) Detection of TUNEL-positive cells within colonic tissues harvested from mice described in (A-D). Scale bar size: 100µm. (F) Quantification of TUNEL-positive cells. Data are represented as mean +/- SEM. Nonparametric unpaired t-test ***p<0.001, n=5.

Article Snippet: Lactobacillus fermentum , ATCC 9338.

Techniques: Bacteria, Irradiation, TUNEL Assay

Journal: iScience

Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

doi: 10.1016/j.isci.2019.01.030

Figure Lengend Snippet: Lactococcus lactis subsp. cremoris ATCC 19257 Protects the Murine Intestine from Dextran Sodium Sulfate (DSS)-Induced Colitis (A) Groups of five 6-week-old male C57/B6 mice were administered either 1 × 10 8 CFU total Lactococcus lactis subsp. cremoris ATCC 19257, or Lactobacillus rhamnosus GG, or vehicle control for 14 days. Feeding of bacteria was ceased before 4% DSS being included in the drinking water (day 0) for 7 days in the indicated groups. Control groups of bacteria-fed mice were subjected to regular water. Measurement of weight loss in mice of the aforementioned groups following commencement of the inclusion of 4% DSS in the drinking water. Data are represented as mean ± SEM. Nonparametric unpaired t test *p < 0.05, n = 5. (B) Disease activity index (DAI) of mice described in (A). Nonparametric unpaired t test *p < 0.05, **p < 0.01, ***p < 0.001. n = 5. (C) Colon length of mice in (A) and (B) at day 10 following commencement of treatment with DSS. Data are represented as mean ± SEM. Nonparametric unpaired t test **p < 0.01, n = 5. (D) Colon weight to length ratio of mice in (A) and (B) at day 10 following commencement of treatment with DSS. Data are represented as mean ± SEM. Nonparametric unpaired t test **p < 0.01, n = 5. (E) Detection of phospho-histone-H3-positive cells in the colonic epithelium of indicated DSS-treated mice at day 10 following commencement of treatment with DSS. (F) Numeration of phospho-histone-H3-positive cells shown in (E). Data are represented as mean ± SEM. Nonparametric unpaired t test *p < 0.05, n = 5 per group. (G) Detection of myeloperoxidase (MPO)-expressing cells in the colonic tissue subepithelial compartments of colonic tissue at day 10 following commencement of treatment with DSS. White arrows point to cells stained positive with anti-Myeloperoxidase/MPO antibody that exhibit characteristic polymorphonuclear morphology of neutrophils. (H) Numeration of MPO-positive cells shown in (G). Data are represented as mean ± SEM. Nonparametric unpaired t test, with p values indicated for each comparison.

Article Snippet: Lactobacillus fermentum , ATCC 9338.

Techniques: Control, Bacteria, Activity Assay, Expressing, Staining, Comparison

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Specificity of the L. Casei Primer Pair Used in This Study

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques: Real-time Polymerase Chain Reaction

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Prevalence of Lactobacillus Species, Detected by Quantitative PCR

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques:

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Quantity of seven Lactobacillus species in the feces of constipated children and healthy controls. Statistical significance of observed differences in the amount of Lactobacillus species between both constipated and healthy groups was measured by the Mann–Whitney U -test. Bars represent standard errors. P < 0.05 was marked with one asterisk (∗), and P < 0.2 with two asterisks (∗∗). One-way ANOVA testing shows the significant differences in quantity (log 10 CFU/gram) between Lactobacillus species.

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques: MANN-WHITNEY

Journal:

Article Title: Persistence of Lactobacillus fermentum RC-14 and Lactobacillus rhamnosus GR-1 but Not L. rhamnosus GG in the Human Vagina as Demonstrated by Randomly Amplified Polymorphic DNA

doi: 10.1128/CDLI.9.1.92-96.2002

Figure Lengend Snippet: RAPD fingerprints of probiotic Lactobacillus strains (L. rhamnosus GR-1 [lane 2], L. fermentum RC-14 [lane 3], and L. rhamnosus GG [lane 4]), culture collection Lactobacillus strains (L. fermentum ATCC 9338 [lane 5], L. rhamnosus ATCC 7469 [lane 6], L. reuteri ATCC 23272 [lane 7], L. plantarum ATCC 14917 [lane 8], L. casei ATCC 393 [lane 9], L. vaginalis NCFB 2810 [lane 10], L. acidophilus ATCC 33199 [lane 11], and L. jensenii ATCC 25258 [lane 12]), and vaginal isolates (L. casei 70 [lane 13] and L. paracasei 55 [lane 14]). Lanes 1 and 15, 100-bp ladders.

Article Snippet: Lactobacillus strains obtained from culture collections ( L. fermentum ATCC 9338, L. rhamnosus ATCC 7469, L. reuteri ATCC 23272, L. plantarum ATCC 14917, L. casei ATCC 393, L. vaginalis NCFB 2810, L. acidophilus ATCC 33199, and L. jensenii ATCC 25258) and some Lactobacillus strains previously isolated from the human urogenital tract ( 23 ) were included in the study for comparative purposes.

Techniques:

Journal:

Article Title: Persistence of Lactobacillus fermentum RC-14 and Lactobacillus rhamnosus GR-1 but Not L. rhamnosus GG in the Human Vagina as Demonstrated by Randomly Amplified Polymorphic DNA

doi: 10.1128/CDLI.9.1.92-96.2002

Figure Lengend Snippet: RAPD fingerprints of L. rhamnosus GR-1 (lane 2), L. rhamnosus GR-1 isolates from a capsule containing the L. rhamnosus GR-1-L. fermentum RC-14 combination (lanes 3 to 5), L. fermentum RC-14 (lane 6), L. fermentum RC-14 isolates from the same L. rhamnosus GR-1-L. fermentum RC-14 capsule (lanes 7 to 9), L. rhamnosus GG (lane 10), and Lactobacillus isolates from a capsule containing the L. rhamnosus GG strain (lanes 11 and 12). Lanes 1 and 13, 100-bp ladders.

Article Snippet: Lactobacillus strains obtained from culture collections ( L. fermentum ATCC 9338, L. rhamnosus ATCC 7469, L. reuteri ATCC 23272, L. plantarum ATCC 14917, L. casei ATCC 393, L. vaginalis NCFB 2810, L. acidophilus ATCC 33199, and L. jensenii ATCC 25258) and some Lactobacillus strains previously isolated from the human urogenital tract ( 23 ) were included in the study for comparative purposes.

Techniques:

Journal:

Article Title: Persistence of Lactobacillus fermentum RC-14 and Lactobacillus rhamnosus GR-1 but Not L. rhamnosus GG in the Human Vagina as Demonstrated by Randomly Amplified Polymorphic DNA

doi: 10.1128/CDLI.9.1.92-96.2002

Figure Lengend Snippet: (A) RAPD fingerprints of L. rhamnosus GR-1 (lane 1), L. fermentum RC-14 (lane 2), and representative lactobacilli isolated from vaginal swabs of subject 210 before (lane 3) and 1 (lanes 4 to 6), 5 (lanes 7 and 8), 12 (lanes 9 and 10), and 18 (lanes 11 and 12) days after vaginal insertion of an L. rhamnosus GR-1-L. fermentum RC-14 capsule for 3 consecutive nights. Lane 13, a 100-bp ladder. (B) PFGE analysis of L. rhamnosus GR-1 (lane 1) and selected vaginal Lactobacillus isolates from subject 207 at 1 day (lanes 2 to 4) and 5 days (lanes 5 to 7) after vaginal insertion of an L. rhamnosus GR-1-L. fermentum RC-14 capsule for 3 consecutive nights. Lane 8, low-range PFGE molecular size markers.

Article Snippet: Lactobacillus strains obtained from culture collections ( L. fermentum ATCC 9338, L. rhamnosus ATCC 7469, L. reuteri ATCC 23272, L. plantarum ATCC 14917, L. casei ATCC 393, L. vaginalis NCFB 2810, L. acidophilus ATCC 33199, and L. jensenii ATCC 25258) and some Lactobacillus strains previously isolated from the human urogenital tract ( 23 ) were included in the study for comparative purposes.

Techniques: Isolation